diff --git a/boards/atxmega-a1u-xpro/Kconfig b/boards/atxmega-a1u-xpro/Kconfig
index efb90396a0..ef85455060 100644
--- a/boards/atxmega-a1u-xpro/Kconfig
+++ b/boards/atxmega-a1u-xpro/Kconfig
@@ -15,6 +15,7 @@ config BOARD_ATXMEGA_A1U_XPRO
     select HAS_PERIPH_CPUID
     select HAS_PERIPH_GPIO
     select HAS_PERIPH_GPIO_IRQ
+    select HAS_PERIPH_I2C
     select HAS_PERIPH_NVM
     select HAS_PERIPH_PM
     select HAS_PERIPH_TIMER
diff --git a/boards/atxmega-a1u-xpro/include/periph_conf.h b/boards/atxmega-a1u-xpro/include/periph_conf.h
index e52ded321f..e4840685cb 100644
--- a/boards/atxmega-a1u-xpro/include/periph_conf.h
+++ b/boards/atxmega-a1u-xpro/include/periph_conf.h
@@ -92,6 +92,26 @@ static const uart_conf_t uart_config[] = {
 #define UART_NUMOF        ARRAY_SIZE(uart_config)
 /** @} */
 
+/**
+ * @name I2C configuration
+ * @{
+ */
+static const i2c_conf_t i2c_config[] = {
+    {
+        .dev                = &TWIC,
+        .pwr                = PWR_RED_REG(PWR_PORT_C, PR_TWI_bm),
+        .sda_pin            = GPIO_PIN(PORT_C, 0),
+        .scl_pin            = GPIO_PIN(PORT_C, 1),
+        .speed              = I2C_SPEED_NORMAL,
+        .int_lvl            = CPU_INT_LVL_LOW,
+    },
+};
+
+#define I2C_0_ISR           TWIC_TWIM_vect
+
+#define I2C_NUMOF           ARRAY_SIZE(i2c_config)
+/** @} */
+
 #ifdef __cplusplus
 }
 #endif
diff --git a/tests/pkg_cryptoauthlib_internal-tests/Makefile.ci b/tests/pkg_cryptoauthlib_internal-tests/Makefile.ci
index 965a0b66a5..130b9e7808 100644
--- a/tests/pkg_cryptoauthlib_internal-tests/Makefile.ci
+++ b/tests/pkg_cryptoauthlib_internal-tests/Makefile.ci
@@ -8,6 +8,7 @@ BOARD_INSUFFICIENT_MEMORY := \
     atmega256rfr2-xpro \
     atmega328p \
     atmega328p-xplained-mini \
+    atxmega-a1u-xpro \
     avr-rss2 \
     blackpill \
     bluepill \