diff --git a/boards/nucleo-f401/Makefile.features b/boards/nucleo-f401/Makefile.features
index 5a6a4e7cae..9b23f31e52 100644
--- a/boards/nucleo-f401/Makefile.features
+++ b/boards/nucleo-f401/Makefile.features
@@ -1,5 +1,6 @@
 # Put defined MCU peripherals here (in alphabetical order)
 FEATURES_PROVIDED += periph_cpuid
+FEATURES_PROVIDED += periph_i2c
 FEATURES_PROVIDED += periph_gpio
 FEATURES_PROVIDED += periph_spi
 FEATURES_PROVIDED += periph_timer
diff --git a/boards/nucleo-f401/include/periph_conf.h b/boards/nucleo-f401/include/periph_conf.h
index 9864839bc2..7464475841 100644
--- a/boards/nucleo-f401/include/periph_conf.h
+++ b/boards/nucleo-f401/include/periph_conf.h
@@ -133,6 +133,35 @@ static const uart_conf_t uart_config[] = {
 /** @} */
 
 
+/**
+ * @name I2C configuration
+ * @{
+ */
+#define I2C_NUMOF           (1U)
+#define I2C_0_EN            1
+#define I2C_IRQ_PRIO        1
+#define I2C_APBCLK          (42000000U)
+
+/* I2C 0 device configuration */
+#define I2C_0_DEV           I2C1
+#define I2C_0_CLKEN()       (RCC->APB1ENR |= RCC_APB1ENR_I2C1EN)
+#define I2C_0_CLKDIS()      (RCC->APB1ENR &= ~(RCC_APB1ENR_I2C1EN))
+#define I2C_0_EVT_IRQ       I2C1_EV_IRQn
+#define I2C_0_EVT_ISR       isr_i2c1_ev
+#define I2C_0_ERR_IRQ       I2C1_ER_IRQn
+#define I2C_0_ERR_ISR       isr_i2c1_er
+/* I2C 0 pin configuration */
+#define I2C_0_SCL_PORT      GPIOB
+#define I2C_0_SCL_PIN       8
+#define I2C_0_SCL_AF        4
+#define I2C_0_SCL_CLKEN()   (RCC->AHB1ENR |= RCC_AHB1ENR_GPIOBEN)
+#define I2C_0_SDA_PORT      GPIOB
+#define I2C_0_SDA_PIN       9
+#define I2C_0_SDA_AF        4
+#define I2C_0_SDA_CLKEN()   (RCC->AHB1ENR |= RCC_AHB1ENR_GPIOBEN)
+/** @} */
+
+
 #ifdef __cplusplus
 }
 #endif