diff --git a/boards/arduino-nano-33-iot/include/periph_conf.h b/boards/arduino-nano-33-iot/include/periph_conf.h
index 7d8275c6af..b41bac9f73 100644
--- a/boards/arduino-nano-33-iot/include/periph_conf.h
+++ b/boards/arduino-nano-33-iot/include/periph_conf.h
@@ -139,7 +139,6 @@ static const uart_conf_t uart_config[] = {
 #define UART_NUMOF          ARRAY_SIZE(uart_config)
 /** @} */
 
-
 /**
  * @name PWM configuration
  * @{
@@ -241,6 +240,18 @@ static const spi_conf_t spi_config[] = {
         .mosi_pad = SPI_PAD_MOSI_0_SCK_3,
         .gclk_src = SAM0_GCLK_MAIN,
     },
+    {
+        .dev = &SERCOM3->SPI,
+        .miso_pin = GPIO_PIN(PA, 19),
+        .mosi_pin = GPIO_PIN(PA, 16),
+        .clk_pin = GPIO_PIN(PA, 17),
+        .miso_mux = GPIO_MUX_D,
+        .mosi_mux = GPIO_MUX_D,
+        .clk_mux = GPIO_MUX_D,
+        .miso_pad = SPI_PAD_MISO_3,
+        .mosi_pad = SPI_PAD_MOSI_0_SCK_1,
+        .gclk_src = SAM0_GCLK_MAIN,
+    },
 };
 
 #define SPI_NUMOF           ARRAY_SIZE(spi_config)